منابع مشابه
Segmented field OFFGEL® electrophoresis.
A multielectrode setup for protein OFFGEL electrophoresis that significantly improves protein separation efficiency has been developed. Here, the electric field is applied by segments between seven electrodes connected in series to six independent power supplies. The aim of this strategy is to distribute evenly the electric field along the multiwell system, and as a consequence to enhance elect...
متن کاملEfficient fractionation and improved protein identification by peptide OFFGEL electrophoresis.
The sample fractionation steps conducted prior to mass detection are critically important for the comprehensive analysis of complex protein mixtures. This paper illustrates the effectiveness of OFFGEL electrophoresis with the Agilent 3100 OFFGEL Fractionator for the fractionation of peptides. An Escherichia coli tryptic digest was separated in 24 fractions, and peptides were identified by rever...
متن کاملAlternative to 2D gel electrophoresis – OFFGEL electrophoresis combined with high-sensitivity on-chip protein detection
Two-dimensional gel electrophoresis (2D-GE) employs isoelectric focusing in the first dimension and a separation of the proteins according to their molecular weights in the second dimension. The gels are then commonly stained using silver stain to visualize the protein pattern. This is a tedious and time-consuming procedure. Here, we demonstrate a combination of two newly developed methods – OF...
متن کاملField gradient electrophoresis.
The class of equilibrium gradient methods utilizes the opposition of two forces, at least one of which changes in magnitude with position, to separate and concentrate analytes. The drawback of many methods of this type is that the production of two opposing forces requires in comparison to standard methods, such as capillary electrophoresis, a relatively complex apparatus. In addition, for tech...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2012
ISSN: 0173-0835
DOI: 10.1002/elps.201200180